CRUNC: a cryopreservation method for unencapsulated gemmae of Marchantia polymorpha

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Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.

The liverwort Marchantia polymorpha L. is one of the key model plants in evo-devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, t...

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The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). ...

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ژورنال

عنوان ژورنال: PeerJ

سال: 2020

ISSN: 2167-8359

DOI: 10.7717/peerj.10174