CRUNC: a cryopreservation method for unencapsulated gemmae of Marchantia polymorpha
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.
The liverwort Marchantia polymorpha L. is one of the key model plants in evo-devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, t...
متن کاملSexual Organs of Marchantia polymorpha
A total of 935 expressed sequence tags (ESTs) from male immature sexual organ were determined, of which 600 ESTs were assembled into 110 non-redundant groups, resulting in 445 unique EST sequences. Of these, 244 sequences shared significant similarities to known nucleotide or amino acid sequences in other organisms. The remaining 201 unique sequences showed no significant matches and thus are l...
متن کاملAgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L.
The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). ...
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Marchantia polymorpha is an extant relative of the earliest terrestrial plants and has attracted a substantial interest as a model organism for evolutionary and developmental studies. Given its relatively simple genome, compact gene families, simple morphology, ease of propagation and transformation, M. polymorpha is becoming a promising platform for plant synthetic biology. Modular genetic par...
متن کاملBiotransformation of progesterone by cultured cells of Marchantia polymorpha.
Cell suspensions of Marchantia polymorpha hydrogenate progesterone to 5alpha-pregnane-3,20-dione. Structure elucidation of the product was achieved by comprehensive NMR analyses.
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ژورنال
عنوان ژورنال: PeerJ
سال: 2020
ISSN: 2167-8359
DOI: 10.7717/peerj.10174